Technical note: Comparison of automated ribosomal intergenic spacer analysis and denaturing gradient gel electrophoresis to assess bacterial diversity in the rumen of sheep1
| Autor: | M. J. Ranilla, Cristina Saro, Ana Cifuentes, Ramon Rosselló-Móra, María Dolores Carro |
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| Rok vydání: | 2014 |
| Předmět: |
2. Zero hunger
0303 health sciences 030306 microbiology Ribosomal Intergenic Spacer analysis food and beverages Forage Technical note General Medicine Biology Microbiology 03 medical and health sciences Rumen Alfalfa hay Genetics Hay Animal Science and Zoology Food science Temperature gradient gel electrophoresis 030304 developmental biology Food Science |
| Zdroj: | Journal of Animal Science. 92:1083-1088 |
| ISSN: | 1525-3163 0021-8812 |
| DOI: | 10.2527/jas.2013-7175 |
| Popis: | The aim of this study was to compare automated ribosomal intergenic spacer analysis (ARISA) and denaturing gradient gel electrophoresis (DGGE) techniques to assess bacterial diversity in the rumen of sheep. Sheep were fed 2 diets with 70% of either alfalfa hay or grass hay, and the solid (SOL) and liquid (LIQ) phases of the rumen were sampled immediately before feeding (0 h) and at 4 and 8 h postfeeding. Both techniques detected similar differences between forages, with alfalfa hay promoting greater (P 0.05) over the postfeeding period were detected by DGGE. The ARISA technique showed lower (P 0.05) in bacterial diversity between both rumen phases were detected by DGGE. Under the conditions of this study, the DGGE was not sensitive enough to detect some changes in ruminal bacterial communities, and therefore ARISA was considered more accurate for assessing bacterial diversity of ruminal samples. The results highlight the influence of the fingerprinting technique used to draw conclusions on factors affecting ruminal bacterial diversity. |
| Databáze: | OpenAIRE |
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