Optimization of cell growth on palmitoyl-hyaluronan knitted scaffolds developed for tissue engineering applications
Autor: | Kristina Nešporová, Jana Batova, Vladimir Velebny, Martina Pospisilova, Vojtech Zapotocky, Hana Vágnerová, Dorothee Daro, Jana Sogorkova, Veronika Stepankova, Garry P. Duffy, Martin Čepa, Martina Hermannová, Jiri Betak |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Scaffold food.ingredient Materials science Biocompatibility Biomedical Engineering 02 engineering and technology Gelatin Biomaterials 03 medical and health sciences chemistry.chemical_compound food Tissue engineering Hyaluronic acid biology Mesenchymal stem cell Metals and Alloys Biomaterial 021001 nanoscience & nanotechnology Fibronectin 030104 developmental biology chemistry Ceramics and Composites Biophysics biology.protein 0210 nano-technology |
Zdroj: | Journal of Biomedical Materials Research Part A. 106:1488-1499 |
ISSN: | 1549-3296 |
Popis: | Polysaccharides meet several criteria for a suitable biomaterial for tissue engineering, which include biocompatibility and ability to support the delivery and growth of cells. Nevertheless, most of these polysaccharides, for example dextran, alginate, and glycosaminoglycans, are highly soluble in aqueous solutions. Hyaluronic acid hydrophobized by palmitic acid and processed to the form of wet-spun fibers and the warp-knitted textile scaffold is water non-soluble, but biodegradable material, which could be used for the tissue engineering purpose. However, its surface quality does not allow cell attachment. To enhance the biocompatibility the surface of palmitoyl-hyaluronan was roughened by freeze drying and treated by different cell adhesive proteins (fibronectin, fibrinogen, laminin, methacrylated gelatin and collagen IV). Except for collagen IV, these proteins covered the fibers uniformly for an extended period of time and supported the adhesion and cultivation of dermal fibroblasts and mesenchymal stem cells. Interestingly, adipose stem cells cultivated on the fibronectin-modified scaffold secreted increasing amount of HGF, SDF-1, and VEGF, three key growth factors involved in cardiac regeneration. These results suggested that palmitoyl-hyaluronan scaffold may be a promising material for various applications in tissue regeneration, including cardiac tissue repair. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1488-1499, 2018. |
Databáze: | OpenAIRE |
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