Analysis of the complete genome sequence of cucumber mosaic virus from Vinca minor and Wisteria sinensis in Iran
| Autor: | Zohreh Moradi, Mohammad Zakiaghl, Mohsen Mehrvar, Sharifeh Dabiri |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Whole genome sequencing Genetics biology Plant Science biology.organism_classification 01 natural sciences Genetic analysis Genome Cucumber mosaic virus 03 medical and health sciences Complete sequence 030104 developmental biology Genetic variability Gene 010606 plant biology & botany Wisteria sinensis |
| Zdroj: | Journal of Plant Pathology. 102:1263-1268 |
| ISSN: | 2239-7264 1125-4653 |
| DOI: | 10.1007/s42161-020-00650-y |
| Popis: | We present here the first complete sequence of cucumber mosaic virus (CMV) from RNA sequencing of a dwarf periwinkle (Vinca minor) and a Wisteria sinensis in Iran by de novo assembly and annotation of contigs. The tripartite genome of CMV-Ir-VM and Ir-WS consists of a 3391-nucleotide (nt) RNA1, a 3038-nt RNA2, and a 2197-nt RNA3 segment. The sequence comparisons and phylogenetic analyses revealed that all three RNA segments of these two isolates belonged to the CMV subgroup II. RNA1, RNA2, and RNA3 of these two isolates, respectively shared 97.61–99.06%, 97.83–98.88%, and 97.19–98.86% nucleotide identities to those of CMV subgroup II isolates. In genetic analysis, the 2b gene revealed more genetic variability (π = 0.2614) compared with other regions. Our results indicated that purifying selection pressure was the major force acting upon the CMV genome, although some positively selected sites were identified for all encoded proteins. |
| Databáze: | OpenAIRE |
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