No Evidence of a Role for Mutations or Polymorphisms of the Follicle-Stimulating Hormone Receptor in Ovarian Granulosa Cell Tumors1
| Autor: | Tom Jobling, Peter J. Fuller, Henry G. Burger, Karen Verity, Pamela Mamers, Yan Shen |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
endocrine system
medicine.medical_specialty Ovarian Granulosa Cell medicine.drug_class Inhibin secretion Endocrinology Diabetes and Metabolism Biochemistry (medical) Clinical Biochemistry Single-strand conformation polymorphism Biology medicine.disease_cause Biochemistry Exon Endocrinology Internal medicine medicine Gonadotropin Carcinogenesis Follicle-stimulating hormone receptor Receptor hormones hormone substitutes and hormone antagonists |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 83:274-279 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/jcem.83.1.4509 |
| Popis: | The molecular pathogenesis of granulosa cell tumors of the ovary is not understood, although recent studies have shown that immunoreactive inhibin secretion by these tumors may be used as a tumor marker. Granulosa cell tumors exhibit many features of normal granulosa cells, including a response to FSH and inhibin secretion. FSH levels are suppressed in patients with inhibin-secreting granulosa cell tumors, suggesting FSH-independent growth of these tumors. Activating mutations of the FSH receptor might, therefore, be involved in tumorigenesis. We sought to identify mutations in the FSH receptor genes of these tumors using PCR to amplify the exon encoding the transmembrane and cytoplasmic domains from the tumor DNA. Analysis of the amplicons for single strand conformational polymorphisms and direct sequencing confirmed a previously reported polymorphism in the C-terminal region of the receptor, but did not identify tumor-specific missense mutations and/or polymorphisms. In addition, ribonucleic acid from 3... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|