Studies on the interaction between a Co(II) complex with salicylaldehyde-aminoacetic acid Schiff base and bovine serum albumin

Autor: H. M. Peng, Sheng-Li Liu, Liang-Cai Yu
Rok vydání: 2013
Předmět:
Zdroj: Russian Journal of Coordination Chemistry. 39:771-776
ISSN: 1608-3318
1070-3284
DOI: 10.1134/s1070328413100114
Popis: A Co(II) complex [Co3(L)4(H2O)6] · 2Cl (I), where L is salicylaldehyde-aminoacetic acid Schiff base, was synthesized and characterized via elemental analysis, UV, and single crystal X-ray crystallography. Complex I crystallizes in the orthorhombic system, space group Pbcn with lattice parameters a = 9.569(4), b = 12.301(5), c = 36.931(14) A, V = 4347(3) A3, Z = 4, ρcalcd = 1.608 mg m−3. At the same time, the binding reaction between complex I and bovine serum albumin (BSA) was studied by fluorescence spectroscopy combined with UV-Vis absorption measurements under simulative physiological conditions. The results indicated that its combination reaction is mainly a static quenching process. Complex I bound BSA with a molar ratio of 1: 1 and the binding constant KA values are 3.86 × 105 L mol−1 (25°C) and 1.17 × 105 L mol−1 (36°C). The shortest binding distance r between the donor BSA and acceptor (complex I) is 2.49 nm, which affirms that complex I has partly inserted into the hydrophobic pocket of BSA.
Databáze: OpenAIRE
Externí odkaz: