Vertical transport of a field-released genetically engineered microorganism through soil

Autor:	Claudia Werner, David E. Nivens, Gary S. Sayler, Steven Ripp
Rok vydání:	2001
Předmět:	biology Water table Ecology Microorganism Soil Science Pseudomonas fluorescens biology.organism_classification Microbiology Soil contamination Bioremediation Lysimeter Environmental chemistry Soil water Environmental science Groundwater
Zdroj:	Soil Biology and Biochemistry. 33:1873-1877
ISSN:	0038-0717
DOI:	10.1016/s0038-0717(01)00120-1
Popis:	The vertical movement of a genetically-engineered bacterium released into a sub-surface soil contained within a lysimeter was investigated. The strain used was Pseudomonas fluorescens HK44, a lux-based microbe capable of bioremediating specific polyaromatic hydrocarbons (PAHs). Strain HK44 was inoculated into a 4 m deep by 2.5 m diameter soil lysimeter outfitted with a hydraulic assembly to control groundwater levels. After 230 days, the lysimeter was subjected to a water table manipulation that resulted in significant transport of HK44 cells to depths 60 cm below the original inoculation zone within 20 days. A lysimeter not exposed to water level fluctuations exhibited no significant vertical transport. These results demonstrate the potential distribution of genetically engineered microorganisms in subsurface soil due to groundwater effects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0cb07f91a6336cbc68045162713a30cb https://doi.org/10.1016/s0038-0717(01)00120-1 Zobrazit plný text záznamu Full Text from ScienceDirect