Effect of Cellular Phospholipase A2Inhibition on Enhancement of Bt Insecticidal Activity
| Autor: | Jiyeong Park, Seonghyeon Eom, Yonggyun Kim, Kunwoo Kim |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Phospholipase A biology Metabolite fungi Spodoptera biology.organism_classification Microbiology chemistry.chemical_compound Phospholipase A2 chemistry Biochemistry Eicosanoid Bacillus thuringiensis Exigua biology.protein lipids (amino acids peptides and proteins) Polyunsaturated fatty acid |
| Zdroj: | Korean journal of applied entomology. 53:271-280 |
| ISSN: | 1225-0171 |
| DOI: | 10.5656/ksae.2014.08.0.027 |
| Popis: | Some bacterial metabolites of Xenorhabdus nematophila (Xn) inhibit phospholipase A2 (PLA2) activity to shutdown eicosanoid biosynthesis in target insects. However, little has been known about the target insect PLA2 of these bacterial metabolites. Eight bacterial metabolites identified in Xn culture broth exhibited significant insecticidal activities against larvae of both lepidopteran species of Plutella xylostella and Spodoptera exigua. Moreover, these bacterial metabolites significantly enhanced insecticidal activities of Bacillus thuringiensis (Bt). To determine target PLA2, we cloned and over-expressed cellular PLA2 (SecPLA2) of S. exigua. Purified SecPLA2 catalyzed phospholipids derived from the fat body and released several polyunsaturated fatty acids. Most Xn metabolites significantly inhibited SecPLA2 activity, but were different in their inhibitory activities. There was a positive correlation between the inhibition of SecPLA2 and the enhancement of Bt insecticidal activity. These results indicate that SecPLA2 is a molecular target inhibited by Xn metabolite. |
| Databáze: | OpenAIRE |
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