Population structure of eulachon (Thaleichthys pacificus) from Northern California to Alaska using single nucleotide polymorphisms from direct amplicon sequencing
| Autor: | Khai D. Le, Krista M. Nichols, Kim Jonsen, Kayla Mohns, Terry D. Beacham, Richard G. Gustafson, Olivia Cornies, John R. Candy, Ben J. G. Sutherland |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0303 health sciences Fish migration Population structure Zoology Single-nucleotide polymorphism Aquatic Science Biology biology.organism_classification 010603 evolutionary biology 01 natural sciences 03 medical and health sciences Amplicon sequencing Thaleichthys Smelt Ecology Evolution Behavior and Systematics 030304 developmental biology |
| Zdroj: | Canadian Journal of Fisheries and Aquatic Sciences. 78:78-89 |
| ISSN: | 1205-7533 0706-652X |
| Popis: | Eulachon (Thaleichthys pacificus), a culturally and ecologically important anadromous smelt (Family Osmeridae), ranges from Northern California to the southeast Bering Sea. In recent decades, some populations have experienced declines. Here we use a contig-level genome assembly combined with previously published restriction site-associated DNA sequencing (RADseq)-derived markers to construct an amplicon panel for eulachon. Using this panel, we develop a filtered genetic baseline of 521 variant loci genotyped in 1989 individuals from 14 populations ranging from Northern California through central Alaska. Consistent with prior genetic studies, the strongest separation occurs among three main regions: from Northern California up to and including the Fraser River; north of the Fraser River to southeast Alaska; and within the Gulf of Alaska. Separating the Fraser River from southern US populations and refining additional substructure within the central coast may be possible in mixed-stock analysis; this will be addressed in future work. The amplicon panel outperformed the previous microsatellite panel and thus will be used in future mixed-stock analyses of eulachon to provide new insights for management and conservation of eulachon. |
| Databáze: | OpenAIRE |
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