Regulation of the Biopotency of Primate Luteinizing Hormone by Gonadotropin-Releasing Hormone in Vitro and in Vivo1
| Autor: | Robert L. Matteri, Jerry A. Robinson, Donald J. Dierschke, N S Rhutasel, William E. Bridson |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
endocrine system
medicine.medical_specialty biology medicine.drug_class Cell Biology General Medicine Gonadotropin-releasing hormone Gonadotropic cell In vitro Endocrinology Reproductive Medicine In vivo biology.animal Internal medicine medicine Primate Gonadotropin Luteinizing hormone hormones hormone substitutes and hormone antagonists Intracellular |
| Zdroj: | Biology of Reproduction. 43:1045-1049 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod43.6.1045 |
| Popis: | Gonadotropin biological/immunological (B/I) ratios have proven to be valuable indicators of the biopotencies of LH and FSH. Observations of rapidly changing LH B/I have been made which suggest the existence of a readily mobilized pool of highly bioactive pituitary gonadotropins. To test this hypothesis, we have examined the role of GnRH in the regulation of LH B/I in vivo and in vitro. The rhesus monkey was used as a model due to its many physiological similarities with the human. A rapid elevation in circulating LH B/I was observed following GnRH administration to male monkeys that was sustained for at least 2 h (15 min; p less than 0.05). The administration of 1 or 10 nM GnRH to cultured pituitary cells was found to significantly increase the B/I of secreted, but not intracellular, LH (p less than 0.05). In unstimulated controls, the B/I of intracellular LH was higher than that of secreted LH (p less than 0.05). These findings are consistent with the notion that a pool of highly active LH exists within the gonadotrophs in primates. One way that GnRH may regulate the bioactivity of circulating LH is by rapidly mobilizing this gonadotropin pool. |
| Databáze: | OpenAIRE |
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