Microcystin production and ecological physiology of Caribbean black band disease cyanobacteria
| Autor: | Laurie L. Richardson, Dina Stanić, Stuart A. Oehrle, Miroslav Gantar |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Cyanobacteria Oscillatoria biology Ecology Toxin Coral Black band disease Microcystin Cyanotoxin 16S ribosomal RNA medicine.disease biology.organism_classification medicine.disease_cause Microbiology chemistry Botany medicine skin and connective tissue diseases Ecology Evolution Behavior and Systematics |
| Zdroj: | Environmental Microbiology. 13:900-910 |
| ISSN: | 1462-2912 |
| DOI: | 10.1111/j.1462-2920.2010.02388.x |
| Popis: | Summary Molecular studies of black band disease (BBD), a coral disease found on tropical and subtropical reefs worldwide, have shown that one 16S rRNA gene sequence is ubiquitous. This sequence has been reported to be a member of the cyanobacterial genus Oscillatoria. In this study, extracts of two cultured laboratory strains of BBD Oscillatoria, and for com- parison two strains of BBD Geitlerinema, all isolated from reefs of the wider Caribbean, were analysed using Ultra-Performance Liquid Chromatography- Tandem Quad Mass Spectrometry (UPLC-MS/MS). The cyanotoxin microcystin-LR (MC-LR) was found in all strains, and one Geitlerinema strain additionally produced MC-YR. Growth experiments that monitored toxin production using enzyme-linked immunosor- bent assay (ELISA) showed that BBD Oscillatoria pro- duced yields of MC-LR equivalent (0.02-0.04 mg g -1 |
| Databáze: | OpenAIRE |
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