Determination of seven pueraria constituents by high performance liquid chromatography and capillary electrophoresis
| Autor: | Ming-Hsing Huang, Shuenn-Jyi Sheu, Lin Ching-Che |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
0303 health sciences Pueraria Chromatography Aqueous solution biology 030309 nutrition & dietetics Chemistry Elution Sodium Potassium 010401 analytical chemistry chemistry.chemical_element biology.organism_classification 01 natural sciences High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound Capillary electrophoresis Phosphoric acid Food Science |
| Zdroj: | Journal of Food and Drug Analysis. 13 |
| ISSN: | 2224-6614 |
| DOI: | 10.38212/2224-6614.2573 |
| Popis: | Puerariae Radix is a commonly used Chinese herbal medicine derived from the dried root of the legume plant, which contains a series of isoflavones as its chief pharmacologically active constituents. Using 7 pueraria constituents as marker substances, a high performance liquid chromatography (HPLC) and a capillary electrophoresis (CE) method were developed to assay the quality of Puerariae Radix within 65 and 50 min, respectively. Extracted samples were analyzed with a Cosmosil 5C18-MS column and eluted with a mixture of potassium dihydrogenphosphate (30 mM, adjusted to pH 3.0 with phosphoric acid) and aqueous acetonitrile solution, or an uncoated capillary (75μm I.D.) and carried with an aqueous solution containing 10 mM sodium dihydrogenphosphate and 40 mM sodium dodecyl sulphate. The relative standard deviation of the marker substances, on the basis of peak-area ratios for 6 replicate injections, was 0.50~0.97% (intraday) and 0.48~0.97% (interday) for HPLC, and 0.88~1.25% (intraday) and 0.85~1.38% (interday) for CE. Both methods yielded accurate results differing by only 5% when applied to the analysis of practical samples of Puerariae Radix. |
| Databáze: | OpenAIRE |
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