| Popis: |
Cryopreservation of gametes is done in all sectors including livestock and humans with minor to major variations in the protocol. However, in fisheries, the protocol has been adapted from the field of livestock and is presently being used for various fish species with little modification in the technology. Hence in this chapter, the protocol used for cryopreservation of fish gametes and the mechanisms and problems involved in it are discussed. Ice formation during freezing is the major factor which decides the cryosurvival of spermatozoa. Hence, special emphasis has been made on the physical and chemical process of ice formation during freezing. |
