1H NMR metabolic profiling of the cryopreserved spermatozoa of the wild endangered Persian sturgeon (Acipenser persicus) with the use of beta-cyclodextrin as an external cryoprotectant

Autor: Alireza Mirvaghefi, Gholamreza Rafiee, Hamid Farahmand, Amirreza Abed-Elmdoust, Ruhollah Rahimi
Rok vydání: 2019
Předmět:
Zdroj: Fish Physiology and Biochemistry. 45:1029-1040
ISSN: 1573-5168
0920-1742
DOI: 10.1007/s10695-019-00615-8
Popis: Persian sturgeon (Acipenser persicus) is an endangered species and genetic resource banking such as gametes and embryo preservation could be one of the most pursued conservation approaches. In this study, deleterious effects of the traditional cryopreservation technique and the effect of different doses of 2-hydroxypropyl-beta-cyclodextrin (HβCD) on thawed spermatozoa quality (motility duration and percentage) of Persian sturgeon (Acipenser persicus) were investigated from metabolic aspects of view. For cryopreserving, semen was diluted with Tris–HCl (100 mM) extenders containing 0, 5, 10, and 15 mM of HβCD in a ratio of 1:1 (semen/extenders). Semen-extenders were filled into 0.5-mL straws and were frozen with the vapor of liquid nitrogen, and then immersed into liquid nitrogen. Cryopreserved spermatozoa were thawed in water baths in 15 s. Two treatments with the highest and the lowest motility percentages (0 and 10 mM of HβCD) were chosen to reveal the extremes of the metabolites change range and were objected to 1H NMR spectroscopy. Univariate (ANOVA) and multivariate (PCA) analysis of the obtained metabolic profiles showed significant changes (P
Databáze: OpenAIRE
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