Study of microsatellite instability in neuroendocrine tumors of pancreas and colon
| Autor: | Maksim N. Duritskiy, Vladimir Trifanov, Natalya N. Timoshkina, Petr N. Gabrichidze, Dmitry Yu. Gvaldin, Sergey V. Sanamyants, Oleg I. Kit, Stanislav Stanislavovich Mezentsev, Evgeniy N. Kolesnikov, Umar Muhmadovich Gaziev, Milana Yu. Mesheryakova |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Cancer Research Pathology medicine.medical_specialty business.industry nutritional and metabolic diseases Microsatellite instability Neuroendocrine tumors medicine.disease digestive system diseases medicine.anatomical_structure Oncology Feature (computer vision) Medicine business Pancreas neoplasms |
| Zdroj: | Journal of Clinical Oncology. 39:e16196-e16196 |
| ISSN: | 1527-7755 0732-183X |
| Popis: | e16196 Background: Microsatellite instability (MSI), as an acquired feature of malignant tumors, is a predictive and prognostic marker. The less aggressive nature of MSI-positive tumors has been associated with high immunogenicity. In the present study, MSI was assessed in NET samples of different localizations. Methods: The sample included 50 patients with a diagnosis of pancreatic NET (G1-G3) and NET of colon (G2-G3). MSI was analyzed by fragment analysis of five microsatellite loci (Bat25, Bat26, NR21, NR24, NR27). The level of MLH1 methylation was detected by pyrosequencing. Results: MSI was noted in 25.8% cases of the NET of colon and in 13.3% cases of the NET of the pancreas. In the case of pancreatic NET, only MSI low level was identified (instability at 1/5 loci), while in the case of NET of colon, most cases were classified as MSI high level. The incidence of MSI of pancreatic NET was consistent with literature data for small intestinal NET (14%), unlike MSI NET and BRAF V600 mutated adenocarcinomas of colon, MSI-positive pancreatic NET were not associated with hypermethylation of the MLH1 promoter. MSI was more often detected in women over 60 years old, at stages of the tumor process without distant metastases (p = 0.49). The sample size did not allow us to determine significant differences in the studied clinical and pathological groups of NETs, however, we note that in all cases of an unfavorable course of the disease (progression, death), was noted MSS status of tumors. Conclusions: Thus, MSI-positive NET of colon resembles MSI-positive adenocarcinomas of colon in frequency and pathogenetic mechanisms, while in terms of the identified frequency of MSI in pancreatic NET resembles the NET of the small intestine. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|