Autor:	A. A. Milyutin, Boris M. Dzhagarov, S. B. Bokut, Nicolai A. Nemkovich, P. A. Kisselev, D. A. Parul, Eugene P. Petrov, A. N. Sobchuk
Rok vydání:	2001
Předmět:	Chemistry Analytical chemistry General Medicine Laser Photochemistry Biochemistry Fluorescence law.invention chemistry.chemical_compound Wavelength Decay time Sulfonate law Potassium phosphate Molecule Binding site
Zdroj:	Biochemistry (Moscow). 66:390-396
ISSN:	0006-2979
DOI:	10.1023/a:1010293211370
Popis:	Binding of 1,8-anilinonaphthalene sulfonate (1,8-ANS) with native human oxyhemoglobin (Hb) in 50 mM potassium phosphate buffer (pH 7.4) was studied by steady state fluorescence spectroscopy and by laser spectrofluorimetry with subnanosecond time resolution. The distribution of fluorescence decay times and parameters of two- and three-exponential deconvolution of the fluorescence kinetics of 1,8-ANS in Hb solution demonstrate that the emission at wavelengths λem of 455-600 nm is not single-exponential and has components with mean decay times
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0c460ae48488fb32143dbf93f1d6125f https://doi.org/10.1023/a:1010293211370 Zobrazit plný text záznamu Full text from SpringerLink