仙鹤草不同提取方式的产物抗脑缺血再灌注损伤实验研究
| Autor: | Huiyuan Zhu, null 朱慧渊，别玉龙，王江，高静，杨冰月，万海同, Yulong Bie, Jiang Wang, Jing Gao, Bingyue Yang, Haitong Wan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty H&E stain Ischemia 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine cardiovascular diseases Nimodipine Ethanol biology business.industry Cerebral infarction General Medicine medicine.disease biology.organism_classification Agrimonia pilosa Hsp70 030104 developmental biology Endocrinology chemistry business Reperfusion injury 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Chinese Medical Sciences Journal. |
| ISSN: | 1001-9294 |
| Popis: | Objective To study the protective effect of agrimony extracts from different extracting methods on cerebral ischemia-reperfusion injury in rats, in order to optimize the extraction scheme of agrimony. Methods Male rats were randomly assigned into seven groups: 1. Sham-operated group, 2. Untreated MCAO group (MCAO), 3. Petroleum ether extract of Agrimonia pilosa treated MCAO group (PEA), 4. Ethyl acetate extract of Agrimonia pilosa treated MCAO group (EAEA), 5. Ethanol extract of Agrimonia pilosa treated MCAO group (EEA), 6. Water extract of Agrimonia pilosa treated MCAO group (WEA), 7. Nimodipine treated MCAO group (NP). Intragastrical drug administration (i.g) was performed at 0 and 6 hours after MCAO. Neurological function tests were performed after reperfusion for 24 hours, then the brain was removed for the evaluations of the cerebral infarction volume (percentage of total brain volume) by immunohistochemistry, histological changes (hematoxylin-eosin staining), Na+/K+-ATPase, Ca2+-ATPase (modified method of Svoboda and Mosinger), mRNA expression of Tumor suppressor gene (P53) and hot shock protein (HSP70) (quantitative real-time PCR). Results The neurological function of MCAO group had significantly higher scores than the sham group (P |
| Databáze: | OpenAIRE |
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