Physical-Chemical and Cytotoxic Properties of Bacillus subtilis IMV B-7724 Extracellular Lectin
| Autor: | A V Chumak, V.O. Shliahovenko, G.V. Didenko, N I Fedosova, N L Cheremshenko, I M Voyeykova, T V Symchych, K.I. Hetman |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
biology Chemistry Lectin General Medicine Bacillus subtilis biology.organism_classification 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Biochemistry Physical chemical Extracellular biology.protein Cytotoxic T cell 030217 neurology & neurosurgery |
| Zdroj: | Mikrobiolohichnyi Zhurnal. 83:39-48 |
| ISSN: | 2616-9258 1028-0987 |
| Popis: | Aim. To study the chemical composition, sugar specificity and physicochemical properties of the extracellular lectin isolated from Bacillus subtilis ІМV В-7724. Methods. Biochemical, spectrophotometric, immunological and cultural methods were used to assess the physicochemical and a number of biological properties of lectin isolated from the culture fluid of bacteria B. subtilis ІМV В-7724. Molecular weight of the lectin was estimated in polyacrylamide gel electrophoresis. Analysis of the elemental composition was done using Perkin-Elmer 2400 CHNS analyzer. Temperature and pH stability of lectin were examined based on residual hemagglutination activity of the lectin. Cytotoxic activity was determined by the MTT-assay. The statistical analysis was made using Student’s t-test. Results. B. subtilis IMV B-7724 lectin is a glycoprotein (protein – 86.0%, carbohydrates – 7.0%) with molecular weight of 18–20 kDa (major). Analysis of the elemental composition revealed that it contains 34.00% of carbon, 7.04% of hydrogen, 16.61% of nitrogen, 42.35% of oxygen. Amino acid composition analysis determined that it is rich in leucine, tyrosine and phenylalanine. The lectin exhibited high sugar-binding specificity toward N-acetylneuraminic and N-glycolylneuraminic acids (minimal inhibitory concentration – 0.3 mM for both sugars). The lectin is heat and acid stable, has long shelf life. Conclusions. These results provide the rationale to pursue further investigation for possible ways and modes of B. subtilis IMB B-7724 lectin application in clinical settings. |
| Databáze: | OpenAIRE |
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