Optimization of On-Site Xylanase Production from Aspergillus niger via Central Composite Design (CCD)

Autor:	Z. X. Ooi, Mahadevan Tarrsini, Yi Peng Teoh, Qi Hwa Ng, Balakrishnan Kunasundari, Siew Hoong Shuit, W. U. Ang, P Y Hoo
Rok vydání:	2020
Předmět:	Central composite design biology Chemistry Aspergillus niger Xylanase biology.organism_classification Pulp and paper industry
Zdroj:	IOP Conference Series: Materials Science and Engineering. 855:012005
ISSN:	1757-899X 1757-8981
DOI:	10.1088/1757-899x/855/1/012005
Popis:	Xylanases have stimulated considerable interest due to their potential application in several industries, especially in the bioethanol sector. Since the vitality of this enzyme is undeniable, this research is focused on optimization of on-site xylanase production from Aspergillus niger (A. niger). This initiative could reduce the dependence of commercial xylanase. Central Composite Design (CCD) was implemented in the process of xylanase production optimization. Incubation temperature and medium pH were two parameters selected to statistically optimized using Response Surface Methodology (RSM) in order to improve the xylanase production. From the data analyzed by Design of Experiment (DoE), maximal xylanase production was predicted to produce under condition of 32.67 °C and pH 4.56 with desirability of 0.936. A validation test with triplicate was done to verify the predicted result. The maximum enzyme activity of 0.5638 U/mL was obtained from the validation test.
Databáze:	OpenAIRE
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