Comparison ofMetarhiziumisolates for biocontrol ofHelicoverpa armigera(Lepidoptera: Noctuidae) in chickpea
| Autor: | Armugam Rajendran, Vandana Ghormade, Santosh B. Chavan, Sanjay K. Patil, Yogesh S. Shouche, S. Kulkarni, Manisha Kapoor, Girish Kulkarni, Mukund V. Deshpande |
|---|---|
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | Biocontrol Science and Technology. 18:809-828 |
| ISSN: | 1360-0478 0958-3157 |
| DOI: | 10.1080/09583150802366475 |
| Popis: | Metarhizium isolates from soil (53) and insect hosts (10) were evaluated for extracellular production of cuticle degrading enzyme (CDE) activities such as chitinase, chitin deacetylase (CDA), chitosanase, protease and lipase. Regression analysis demonstrated the relation of CDE activities with Helicoverpa armigera mortality. On basis of this relation, ten isolates were selected for further evaluation. Subsequently, based on LT50 of the 10 isolates towards H. armigera, five isolates were selected. Out of these five isolates, three were selected on the basis of higher conidia production (60–75 g/kg rice), faster sedimentation time (ST50) (2.3–2.65 h in 0.1% (w/v) Tween 80) and lower LC50 (1.4–5.7×103 conidia/mL) against H. armigera. Finally, three Metarhizium isolates were selected for the molecular fingerprinting using ITS sequencing and RAPD patterning. All three isolates, M34412, M34311 and M81123, showed comparable RAPD patterns with a 935G primer. These were further evaluated for their field p... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|