Rejuvenation of Sequoia sempervirens by Repeated Grafting of Shoot Tips onto Juvenile Rootstocks in Vitro

Autor:	Bau-Lian Huang, Suwenza Lius, Richard Van Gundy, Toshio Murashige, Li-Chun Huang, El Fatih M. Mahdi
Rok vydání:	1992
Předmět:	Physiology fungi Sequoia food and beverages Plant Science Biology biology.organism_classification Cutting chemistry.chemical_compound Basal shoot chemistry Callus Botany Shoot Genetics Juvenile Rootstock Gibberellic acid
Zdroj:	Plant Physiology. 98:166-173
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.98.1.166
Popis:	Repeated grafting of 1.5-centimeter long shoot tips from an adult Sequoia sempervirens tree onto fresh, rooted juvenile stem cuttings in vitro resulted in progressive restoration of juvenile traits. After four successive grafts, stem cuttings of previously adult shoots rooted as well, branched as profusely, and grew with as much or more vigor as those of seedling shoots. Reassays disclosed retention for 3 years of rooting competence at similar levels as originally restored. Adventitious shoot formation was remanifested and callus development was depressed in stem segments from the repeatedly grafted adult. The reversion was associated with appearance and disappearance of distinctive leaf proteins. Neither gibberellic acid nor N(6)-beneyladenine as nutrient supplements duplicated the graft effects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0c0775960c049c6ee6cde5cdc44504d0 https://doi.org/10.1104/pp.98.1.166 Zobrazit plný text záznamu