Optimization of a multiresidue and multiclass analysis method for anabolic agents and β2-agonists in bovine urine by GC-MS/MS
| Autor: | Francisco Radler de Aquino Neto, Celeste Yara dos Santos Siqueira, Kelly Thaynan J. Santos, Vania Cristina da Silva Gonçalves, Virgínia Verônica de Lima, Monica Costa Padilha, Nathália Oliveira Cavalcanti Zúniga |
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| Rok vydání: | 2017 |
| Předmět: |
Analyte
Chromatography Resolution (mass spectrometry) Anabolism Chemistry 010401 analytical chemistry Extraction (chemistry) 02 engineering and technology 021001 nanoscience & nanotechnology Tandem mass spectrometry 01 natural sciences Anabolic Agents 0104 chemical sciences Analytical Chemistry Gas chromatography Gas chromatography–mass spectrometry 0210 nano-technology Spectroscopy |
| Zdroj: | Microchemical Journal. 133:551-555 |
| ISSN: | 0026-265X |
| DOI: | 10.1016/j.microc.2017.03.033 |
| Popis: | A simple multiresidue and multiclass procedure is described for the simultaneous determination of anabolic agents and β2-agonists from 4 classes of prohibited substances of illegal use in food-producing animals, according to Council Directive 96/23/EC. The method involves analysis of hydrolysed urinary anabolic compounds using liquid–liquid extraction, with subsequent conversion to trimethylsilylether derivatives that are analysed by GC-MS/MS. The oven was set to an initial temperature of 140°C, was increased at a rate of 10°C/min to 300°C, and was held 3 min using an Ultra-1 capillary column. Optimization tests demonstrated good peak resolution and shape for all compounds. A pre-validation study was conducted to obtain an analytical test curve, which showed adequate results for all analytes. Cochran's test (C calculated |
| Databáze: | OpenAIRE |
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