| Autor: |
Tsutomu Maruyama, Yasuo Kudoh, Makoto Ohashi, Katsutoshi Mise, Michiko Miyahara |
| Rok vydání: |
1990 |
| Předmět: |
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| Zdroj: |
Microbial Toxins in Foods and Feeds ISBN: 9781461279167 |
| DOI: |
10.1007/978-1-4613-0663-4_12 |
| Popis: |
Restriction endonucleases have been employed as an extremely important tool in recombinant DNA technology. To date, the occurrence of more than 100 restriction endonucleases with different specificities has been reported.2,8 Restriction endonucleases have been screened in this laboratory for pathogenic bacteria belonging to the Enterobacteriaceae in the hope that: (i) the detection of specific restriction endonuclease is found at a high frequency in the species; and (ii) new restriction endonucleases with novel specificities might be found in pathogenic bacteria, since screening of restriction endonucleases have been rarely carried out. Here, we report that four clinically important food-poisoning bacteria produce specific restriction endonucleases at high frequencies. Some of these are expected to be useful for recombinant DNA technology after cloning of their gene into Escherichia coli K-12. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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