| Autor: |
Zen C. Chung, J. R. Tzou, Sung M. Wang, Norman C. Li, Yee-Hsiung Chen, Min N. Chen |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Journal of Inorganic Biochemistry. 29:231-239 |
| ISSN: |
0162-0134 |
| DOI: |
10.1016/0162-0134(87)80030-2 |
| Popis: |
Several derivatives of hemocyanin from Taiwan snails ( Achatina fulica ) have been prepared. The reconstituted protein (R-HcO 2 ) has lower Cu content, lower circular dichroism intensity, and higher fluorescence intensity than native oxyhemocyanin (HcO 2 ). The Co(II) derivative (CoHc) does not take up molecular oxygen and only 50% of the total sites for Cu in native hemocyanin is taken up by Co. The half-apo derivative (half-apo-Hc) contains a single Cu per active site. Divalent cations quench the tryptophan fluorescence in the hemocyanin species and also quench the fluorescence from Tb 3+ bound to the protein. The collisional quenching constants decrease in the order Co 2+ > Mn 2+ > Ca 2+ . The static component is negligible. For carboxy hemocyanin (HcCO), fluorescence originates from a Cu(I) CO complex and was used to study reaction of Hc CO with CN − . |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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