Covalent immobilization of trypsin onto thermo-sensitive poly(N-isopropylacrylamide-co-acrylic acid) microspheres with high activity and stability
| Autor: | Guang Li, Yuxia Wang, Guanghui Ma, Enping Lai, Yi Wei |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Chromatography Polymers and Plastics Immobilized enzyme 02 engineering and technology General Chemistry Buffer solution 010402 general chemistry 021001 nanoscience & nanotechnology Trypsin 01 natural sciences Michaelis–Menten kinetics 0104 chemical sciences Surfaces Coatings and Films chemistry.chemical_compound chemistry Covalent bond Materials Chemistry Poly(N-isopropylacrylamide) medicine 0210 nano-technology Membrane emulsification Nuclear chemistry Acrylic acid medicine.drug |
| Zdroj: | Journal of Applied Polymer Science. 133 |
| ISSN: | 0021-8995 |
| Popis: | Poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AA)) microspheres with a high copolymerized AA content were fabricated using rapid membrane emulsification technique. The uniform size, good hydrophilicity, and thermo sensitivity of the microspheres were favorable for trypsin immobilization. Trypsin molecules were immobilized onto the microspheres surfaces by covalent attachment. The effects of various parameters such as immobilization pH value, enzyme concentration, concentration of buffer solution, and immobilization time on protein loading amount and enzyme activity were systematically investigated. Under the optimum conditions, the protein loading was 493 +/- 20 mgg(-1) and the activity yield of immobilized trypsin was 155%+/- 3%. The maximum activity (V-max) and Michaelis constant (K-m) of immobilized enzyme were found to be 0.74 Ms-1 and 0.54 mM, respectively. The immobilized trypsin showed better thermal and storage stability than the free trypsin. The enzyme-immobilized microspheres with high protein loading amount still can show a thermo reversible phase transition behavior. The research could provide a strategy to immobilize enzyme for application in proteomics. (c) 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43343. |
| Databáze: | OpenAIRE |
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