HER2+breast cancer cells undergo apoptosis upon exposure to tannic acid released from remodeled cross-linked collagen type I
Autor: | Brian W. Booth, Lauren G. Jordan |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Materials science medicine.drug_class Cell Biomedical Engineering Matrix (biology) Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Breast cancer Tannic acid medicine skin and connective tissue diseases Triple-negative breast cancer Metals and Alloys Caspase Gene medicine.disease Molecular biology 030104 developmental biology medicine.anatomical_structure Biochemistry chemistry Apoptosis Estrogen 030220 oncology & carcinogenesis Ceramics and Composites |
Zdroj: | Journal of Biomedical Materials Research Part A. 106:26-32 |
ISSN: | 1549-3296 |
Popis: | Tannic acid (TA) is a naturally occurring polyphenol that cross-links collagen type I and possesses anticancer potential. In previous studies, we demonstrated the increased sensitivity of estrogen receptor-positive (ER+ ) breast cancer cells to TA as opposed to triple negative breast cancer cells and normal human breast epithelial cells. In the current study, human pre-adipocytes and HER2+ breast cancer cells were grown on TA cross-linked collagen type I beads. Cell attachment, growth, and proliferation of the cells result in remodeling of the collagen matrix and release of the cross-linking TA. TA concentrations in the conditioned media were determined. Induced apoptosis of cells grown on the TA cross-linked collagen type I beads was imaged and quantified. Viability of HER2+ breast cancer cells and normal breast epithelial cells after exposure TA released from bead remodeling was quantified. Caspase gene expression and protein expression were evaluated. HER2+ breast cancer cells underwent caspase-mediated apoptosis in response to TA exposure. TA-induced apoptosis in a concentration- and time-dependent manner, with HER2+ breast cancer cells demonstrating an increased sensitivity to the TA effects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 26-32, 2018. |
Databáze: | OpenAIRE |
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