Development and validation of an ion-exchange chromatography method for heparin and its impurities in heparin products

Autor:	Johan Viaene, Brompoj Prutthiwanasan, Sumate Thiangthum, Wolfgang Buchberger, Leena Suntornsuk, Yvan Vander Heyden
Rok vydání:	2014
Předmět:	Detection limit Tris Analyte Chromatography Sodium Ion chromatography chemistry.chemical_element Filtration and Separation Heparin Dermatan sulfate Analytical Chemistry chemistry.chemical_compound chemistry medicine Chondroitin sulfate medicine.drug
Zdroj:	Journal of Separation Science. 37:3195-3204
ISSN:	1615-9306
Popis:	An anion-exchange liquid chromatography method for the determination of heparin and its impurities (dermatan sulfate and oversulfated chondroitin sulfate) was developed using chemometric-assisted optimization, including multivariate experimental design and response surface methodology. The separation of heparin, dermatan sulfate, and oversulfated chondroitin sulfate (Rs above 2.0) was achieved on a Dionex RF IC IonPac AS22 column with a gradient elution of 10-70% of 2.5 M sodium chloride and 20 mM Tris phosphate buffer (pH 2.1) at a flow rate of 0.6 mL/min and UV detection at 215 nm. Method validation shows good linearity (r > 0.99), acceptable precision (%relative standard deviations
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0bb385efa7bdd648199602fce28636f0 https://doi.org/10.1002/jssc.201400348 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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