A simple neridronate-based surface coating strategy for upconversion nanoparticles: highly colloidally stable 125I-radiolabeled NaYF4:Yb3+/Er3+@PEG nanoparticles for multimodal in vivo tissue imaging
| Autor: | Pavel Švec, Volodymyr Lobaz, Ondřej Sedláček, Pavla Francová, Věra Kolářová, Martin Hrubý, Daniel Horák, Olga Janoušková, Luděk Šefc, Uliana Kostiv, Jan Kučka |
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| Rok vydání: | 2017 |
| Předmět: |
Biodistribution
Materials science Biocompatibility Nanoparticle Nanotechnology 02 engineering and technology Photothermal therapy 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Photon upconversion 0104 chemical sciences Surface coating chemistry.chemical_compound chemistry PEG ratio General Materials Science 0210 nano-technology Ethylene glycol Nuclear chemistry |
| Zdroj: | Nanoscale. 9:16680-16688 |
| ISSN: | 2040-3372 2040-3364 |
| DOI: | 10.1039/c7nr05456d |
| Popis: | In this report, monodisperse upconversion NaYF4:Yb3+/Er3+ nanoparticles with superior optical properties were synthesized by the oleic acid-stabilized high-temperature co-precipitation of lanthanide chlorides in octadec-1-ene as a high-boiling organic solvent. To render the particles with biocompatibility and colloidal stability in bioanalytically relevant phosphate buffered saline (PBS), they were modified by using in-house synthesized poly(ethylene glycol)-neridronate (PEG-Ner), a bisphosponate. The NaYF4:Yb3+/Er3+@PEG nanoparticles showed excellent long-term stability in PBS and/or albumin without any aggregation or morphology transformation. The in vitro cytotoxicity of the nanoparticles was evaluated using primary fibroblasts (HF) and a cell line derived from human cervical carcinoma (HeLa). The particles were subsequently modified by using Bolton–Hunter-hydroxybisphosphonate to enable radiolabeling with 125I for single-photon emission computed tomography/computed tomography (SPECT/CT) bimodal imaging to monitor the biodistribution of the nanoparticles in non-tumor mice. The bimodal upconversion 125I-radiolabeled NaYF4:Yb3+/Er3+@PEG nanoparticles are prospective for near-infrared (NIR) photothermal/photodynamic and SPECT/CT cancer theranostics. |
| Databáze: | OpenAIRE |
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