| Popis: |
The work is devoted to the study of methods of immobilization of peroxidase (KF 1.11.1.7) in the polymer matrix of acetylcellulose and polyacrylamide, as well as in the gel structure of polyvinylpyrolidone. Modifying agents were used to efficiently incorporate the enzyme into the polymer grid. such as chitosan, glutaric dialdehyde. 2,2-Azino-bis-(3-ethylbenzthyosoline-6-sulfonic acids) diammonium salt (ABTS) was used as a substrate in the study of the activity of the immobilized enzyme obtained. Experimental data showed a high activity of the enzyme included in the matrix of polyvinylpyrrolidone: the degree of binding was 85%, the activity expressed by the Michaelis constant Km=14×10−3 mmol/L. The results obtained allow us to recommend the use of enzymes immobilized in polymer matrices of polyvinylpyrrolidone, acetylcellulose, polyacrylamide to create highly sensitive electrodes. |
