Rejection of single stranded and double stranded DNA by porous hollow fiber membranes

Autor:	Gen Ishikawa, Akon Higuchi, Mariko Hara, Hiroo Nakano, Kenichiro Kato, Sei-ichi Manabe, Sakae Satoh, Tetsuo Sato
Rok vydání:	1996
Předmět:	Molecular mass Chemistry Microfiltration Regenerated cellulose Filtration and Separation Permeation Biochemistry chemistry.chemical_compound Membrane Biophysics General Materials Science Fiber Physical and Theoretical Chemistry Cellulose DNA
Zdroj:	Journal of Membrane Science. 116:191-197
ISSN:	0376-7388
DOI:	10.1016/0376-7388(96)00051-8
Popis:	The permeation of DNA through regenerated cellulose hollow fibers that have a mean pore diameter of 15 nm was investigated in the absence of protein at a transmembrane pressure of 200 mmHg at 25°C. The membranes can reject both double stranded DNA and single stranded DNA to some extent when the DNA concentration of the feed solution is 10–100 ppm. It was found that single stranded DNA can permeate through the membranes preferentially over double stranded DNA. This is explained by the higher flexibility of the single stranded DNA over the double stranded DNA and is not mainly due to a different distribution of molecular weights between the single stranded DNA and the double stranded DNA. The circular dichroism spectra of feed and permeate solutions suggest that the DNA was not denatured by permeation through the membranes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0b99f12e6cf89d0ad27b53c788297fc9 https://doi.org/10.1016/0376-7388(96)00051-8 Zobrazit plný text záznamu Full Text from ScienceDirect