Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume

Autor:	Fábio Gelape Faleiro, R. P. Sassaki, R. M. S. Resende, Maria Imaculada Zucchi, Anete Pereira de Souza, Thais Helena Silva Ferreira, Lucimara Chiari, L. Jungmann, C. T. Karia, M. O. Santos
Rok vydání:	2009
Předmět:	Germplasm Genetic diversity biology Stylosanthes Botany Genetics Biodiversity Microsatellite Polymorphic Microsatellite Marker Locus (genetics) Allele biology.organism_classification Ecology Evolution Behavior and Systematics
Zdroj:	Conservation Genetics Resources. 1:481-485
ISSN:	1877-7260 1877-7252
Popis:	Microsatellites were developed for Stylosanthes macrocephala, aiming at developing tools for studying the genetic diversity of this species. A total of 13 polymorphic microsatellite markers were isolated from a S. macrocephala enriched genomic library. The isolated microsatellites were characterized in 20 accessions of the S. macrocephala germplasm collection belonging to the Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) Cerrados. The number of alleles per locus varied from 2 to 11, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.25 and 0.05 to 0.90, respectively. Cross-amplification of the S. macrocephala polymorphic microsatellites was evaluated in three other Stylosanthes species. The microsatellites reported herein are the first set of microsatellite markers developed for S. macrocephala and are potentially useful for further studies on genetic diversity, conservation and breeding of this species.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0b847b4e4c6ba61f420fd9d9ba7e590e https://doi.org/10.1007/s12686-009-9112-x Zobrazit plný text záznamu