Faster radioimmunoassay of angiotensin I at 37 degrees C

Autor:	L Puutula, Frej Fyhrquist
Rok vydání:	1978
Předmět:	medicine.medical_specialty Chromatography Plasma samples Chemistry Isoelectric focusing Biochemistry (medical) Clinical Biochemistry Radioimmunoassay Neomycin Plasma renin activity Standard curve Endocrinology Internal medicine Renin–angiotensin system medicine Incubation medicine.drug
Zdroj:	Clinical Chemistry. 24:115-118
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/24.1.115
Popis:	We applied a 1-h radioimmunoassay incubation at 37 degrees C in determining generated angiotensin I in an assay for plasma renin activity. Under these nonequilibrium conditions, 26% of the 125I-labeled angiotensin I was bound at zero dose of unlabeled angiotensin, as compared to 57% at equilibrium after 18 h at 4 degrees C. Sensitivity and useful range for the standard curve remained unchanged. Blanks were not altered. There was a good (r = .971) correlation between renin values in 120 plasma samples from hypertensive patients as measured with both procedures. With isoelectric focusing, we detected no damage to the labeled angiotensin I during incubation for 1 h at 37 degrees C in the presence of diluted plasma, disodium ethylenediaminetetraacetate, hydroxyquinoline, and neomycin. Analytical recovery of unlabeled angiotensin I added to the assay mixture was 98 +/- 2.3% (mean +/- SD). We conclude that our incubation conditions allow rapid and accurate assay of plasma renin activity to be completed in one working day.
Databáze:	OpenAIRE
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