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The work is devoted to the study of the effect of a low-frequency magnetic field of low intensity on microorganisms. We proceeded from the fundamental fact that the Earth's magnetic field (natural electromagnetic background) is the most important environmental factor affecting all vital processes of living organisms. It is important to realize that in the modern world the negative anthropogenic impact on the natural electromagnetic background has significantly increased due to the rapidly growing number of sources of technogenic fields. The relevance of research is due to the great scientific and practical interest in the issues of survival and growth of colonies of microorganisms under the influence of a magnetic field. We set ourselves the goal of studying the action, in particular, of a low-frequency magnetic field of low intensity. Our task was to find out how the key characteristics of the culture of microorganisms (growth and survival) change under the influence of different field values. We used the device "Biostimulus 1", developed by us at IEINT, which makes it possible to influence biological material with a low-frequency magnetic field of low intensity at specified values. The microbiological material was investigated - Streptomyce canosus CNMN-Ac-02 in the form of an aqueous suspension; an aqueous suspension pounded on the surface of agar and in lyophilic form. Exposure time: 10, 15 and 20 minutes. As a result, it was found that when an aqueous suspension of Streptomyce canosus CNMN-Ac-02 was treated with a magnetic field for 10 and 15 minutes, the appearance of colonies of 2 types with mycelium of different color and size was observed. With an exposure of 20 min. colonies of 4 types arise with the peculiarity of some colonies to release a pigment of a new color into the medium. In the case of a culture in the form of a suspension pounded on agar, similar results were obtained. The survival rate of the S.canosus CNMN-Ac-02 streptomycete strain after exposure to a magnetic field depends on the treatment time, as well as on the state of the culture (dry lyophilized culture or its aqueous suspension). |
