Gel permeation chromatographic characterization of sodium hyaluronate and its fractions prepared by ultrasonic degradation

Autor:	Ivan Novák, L. Šoltés, Peter Chabrecek, Z. Kállay
Rok vydání:	1990
Předmět:	Chromatography Sonication Organic Chemistry Clinical Biochemistry Dispersity Size-exclusion chromatography Sodium hyaluronate Permeation Biochemistry Analytical Chemistry Gel permeation chromatography chemistry.chemical_compound chemistry Hyaluronic acid Degradation (geology)
Zdroj:	Chromatographia. 30:201-204
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02274547
Popis:	High-molecular-weight sodium hyaluronate isolated from rooster combs was degraded by ultrasonication. The molecular weight of hyaluronate and its polydispersity was determined by gel-permeation chromatography. During 75-min treatment the molecular weight value decreased from 1.39×106 Da to 2.25×105 Da while the polydispersity of the molecular weight increased from 1.29 to 2.36. The reciprocal value of the square of the hyaluronate's molecular weight was linearly proportional to the time of ultrasonication.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0b5600e6bda16cea26f2b8595ddb7e97 https://doi.org/10.1007/bf02274547 Zobrazit plný text záznamu Full text from SpringerLink