Recombinant LipL32 Based Enzyme Linked Immunosorbent Assay for Seroprevalence Studies of Leptospirosis in Livestock Farmers

Autor: Gitanjali Sarangi, Suresh Chandra Das, Deepak Pal, Ashok Kumar, Sabrinath Thankpan, S. K. Behera, Pallab Chaudhary, Sasmita Khatua
Rok vydání: 2014
Předmět:
Zdroj: Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 85:873-877
ISSN: 2250-1746
0369-8211
DOI: 10.1007/s40011-014-0421-9
Popis: Leptospirosis is one of the most widespread zoonosis, where humans are the accidental hosts and get infected by urine of rodents and carrier mammals. In general, synchronized study on leptospirosis is scanty among occupational exposed groups in eastern India. Therefore, the present investigation was undertaken to study seroprevalence of leptospirosis among livestock handlers using recombinant LipL32 enzyme linked immunosorbent assay (rLipL32 IgG ELISA). A total of 155 sera sample were collected randomly from people having history of animal rearing and subjected to microscopic agglutination test (MAT) and rLipL32 IgG ELISA. Of the sera sample tested, 22 (14 %) and 25 (16 %) were found positive by both MAT and rLipL32 with Grippotyphosa as the predominant serogroup (10 %), followed by Icterohaemorrhagiae (6.5 %), Australis (3 %), and Hardjo (1 %). Sensitivity and specificity of rLipL32 IgG ELISA was found to be 100 and 97 % respectively in comparison to MAT. The presence of Leptospira antibody in human subjects associated with animal rearing is an important concern to public health personnel and sero-epidemiological survey is an important tool to lower the number of outbreaks.
Databáze: OpenAIRE
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