Methods for the Study of Renal Fibrosis in Human Pluripotent Stem Cell-Derived Kidney Organoids
| Autor: | Dario R. Lemos, Alejandro Moran-Horowich |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Kidney Cell signaling Stromal cell urogenital system 030232 urology & nephrology Nephron Biology medicine.disease Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Fibrosis medicine Organoid Renal fibrosis Induced pluripotent stem cell |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071613818 |
| Popis: | The mechanisms of kidney injury and fibrosis can now be studied using kidney organoids derived from human pluripotent stem cells (hPSCs). Mature kidney organoids contain nephrons and stromal cells with fibrogenic potential, spatially organized in a manner that resembles the anatomy of the kidney. Organoid nephron damage and interstitial fibrosis can be induced under well-controlled experimental conditions in vitro, making this an ideal system for the study of tissue-intrinsic cell signaling and intercellular crosstalk mechanisms in the absence of systemic signals and immune cells that are present in vivo. Here we describe methods for the generation of kidney organoids from a widely used hPSC line, and for the induction and analysis of nephron damage and interstitial fibrosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|