The Escherichia coli multiple antibiotic resistance activator protein represses transcription of the lac operon

Autor:	Rachel A. Kettles, Lisa E Lamberte, David C. Grainger, Anna Lankester, Shafayeth Ahmed
Rok vydání:	2019
Předmět:	Genetics 0303 health sciences 030306 microbiology Operon lac operon Biology medicine.disease_cause Biochemistry 03 medical and health sciences Transcription (biology) medicine bacteria Efflux Escherichia coli Psychological repression Gene Transcription factor 030304 developmental biology
Zdroj:	Biochemical Society Transactions. 47:671-677
ISSN:	1470-8752 0300-5127
DOI:	10.1042/bst20180498
Popis:	In Escherichia coli, the marRAB operon is a determinant for antibiotic resistance. Such phenotypes require the encoded transcription factor MarA that activates efflux pump expression. To better understand all genes controlled by MarA, we recently mapped binding of the regulator across the E. coli genome. As expected, many MarA targets were adjacent to genes encoding stress response systems. Surprisingly, one MarA-binding site overlapped the lac operon regulatory region. Here, we show that MarA specifically targets this locus and can block transcription of the lac genes. Repression is mediated by binding of MarA to a site overlapping the lacP1 promoter −35 element. Control of the lac operon by MarA does not impact antibiotic resistance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0b0462f5901980cffe02cbd55aa3af5e https://doi.org/10.1042/bst20180498 Zobrazit plný text záznamu