High Resolution Structure Determination of Biological Macromolecules

Autor:	F.P. Ottensmeyer, D.P. Bazett-Jones, A.P. Korn
Rok vydání:	1978
Předmět:	0303 health sciences 03 medical and health sciences Materials science High resolution Nanotechnology 02 engineering and technology General Medicine 021001 nanoscience & nanotechnology 0210 nano-technology 030304 developmental biology Macromolecule
Zdroj:	Proceedings, annual meeting, Electron Microscopy Society of America. 36:147-159
ISSN:	2690-1315 0424-8201
DOI:	10.1017/s0424820100069946
Popis:	The electron microscopic extraction of information from biological specimens has always been a compromise among at least four factors: resolution, contrast, radiation damage and signal-to-noise ratio. To overcome the notoriously low contrast of most biological specimens examined in bright field, heavy atom staining or shadowing has been used as a seemingly inevitable necessity. Three benefits result, the most important being that one can now observe structure. In addition, however, the specimen, or rather its heavy atom shell, is relatively radiation resistant and can be examined at length. Nevertheless,Williams and Fisher (1970) have indicated the importance of low specimen exposure even under these conditions.
Databáze:	OpenAIRE
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