Antibiotic susceptibility testing ofCandida albicans by flow cytometry
| Autor: | R. S. Pore |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Antifungal drug
General Medicine Pharmacology Biology biology.organism_classification Applied Microbiology and Biotechnology Microbiology Corpus albicans Flucytosine chemistry.chemical_compound Minimum inhibitory concentration chemistry medicine Ketoconazole Propidium iodide Growth inhibition Candida albicans medicine.drug |
| Zdroj: | Current Microbiology. 20:323-328 |
| ISSN: | 1432-0991 0343-8651 |
| DOI: | 10.1007/bf02091913 |
| Popis: | A flow cytofluorometric susceptibility test (FCST) for in vitro antifungal drug testing ofCandida albicans was developed. Membrane damage was indicated by increased cellular fluorescence owing to propidium iodide or rose bengal uptake. Ketoconazole caused an exponential dose-response effect, best defined by Emax, at therapeutically achievable concentrations (0.02–0.2 µg/ml). This effect was not comparable to the conventional minimum inhibitory concentration (MIC) effect elicited by higher antibiotic concentrations. Amphotericin B, on the other hand, did not elicit Emax, but caused a >1 log dose-response effect which did correspond to the MIC. 5-Fluorocytosine susceptibility was also measurable. Other cytometric data indicating abnormal growth and growth inhibition, as well as conventional growth inhibition testing, confirmed that the 10-h FCST measured useful parameters of in vitro susceptibility. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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