Preparation and biological evaluation of radiogallium labeled glucagon for SPECT imaging
| Autor: | Alireza Doroudi, Mahdokht Jouiaei, Javad Garousi, Sedigheh Moradkhani, Amir Reza Jalilian |
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| Rok vydání: | 2010 |
| Předmět: |
Biodistribution
Kidney Chemistry business.industry Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Spleen Pollution Glucagon Molecular biology Analytical Chemistry medicine.anatomical_structure Nuclear Energy and Engineering TRACER Spect imaging medicine Radiology Nuclear Medicine and imaging Pancreas Nuclear medicine business Receptor Spectroscopy |
| Zdroj: | Journal of Radioanalytical and Nuclear Chemistry. 285:555-561 |
| ISSN: | 1588-2780 0236-5731 |
| DOI: | 10.1007/s10967-010-0555-z |
| Popis: | In this work, recently prepared 67Ga-labeled glucagon (67Ga-DTPA-GCG) for imaging studies (radiochemical purity >94%; HPLC, S.A. 296–370 GBq/mM) was used in biological studies. The wild-type rat biodistribution results, 2 h post injection, demonstrated high tissue:muscle ratios for target tissues (liver, kidney, heart, spleen, fat intestine stomach and pancreas), 234, 18.45, 7.12, 1.75, 128.7, 4.9, 6.3 and 1.11, respectively. The tracer binding capacity using freshly prepared rat brain homogenate demonstrated significant specific binding of the tracer to neuronal GCG receptors (67Ga-DTPA-GCG/67Ga:3 and 67Ga-DTPA-GCG/67GaDTPA:2.2 at 90 min). SPECT images also demonstrated target specific binding of the tracer at 4 h. The data suggests the tracer is accumulated in GCGR rich tissues 2–4 h post injection, suggesting potentials of the tracer for future imaging studies in glocagonoma models. |
| Databáze: | OpenAIRE |
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