Two-Color Fluorescence in Flow Cytometry DNA Sizing: Identification of Single-Molecule Fluorescent Probes

Autor:	and Bart G. de Grooth, Jan Greve, Juleon M. Schins, A. Agronskaia
Rok vydání:	1999
Předmět:	medicine.diagnostic_test Chemistry technology industry and agriculture Analytical chemistry Laser Fluorescence Analytical Chemistry law.invention Flow cytometry chemistry.chemical_compound Relative fluorescence units law Excited state Nucleic acid medicine Molecule DNA
Zdroj:	Analytical Chemistry. 71:4684-4689
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac990422v
Popis:	We have built a two laser flow cytometer for ultrasensitive fluorescence measurements. The instrument is used for two-parameter analysis of DNA fragments. Total DNA content of each fragment (proportional to its size) is measured using TOTO-1 excited at 514 nm. Specific targets on the DNA are labeled with Cy5 and excited at 647 nm. The sensitivity of the instrument allows the detection of targets at the single molecule level. The signal-to-noise ratio for a single streptavidin-conjugated Cy5 molecule (about four Cy5 molecules) is 7.5. The SNR for a single Cy5 molecule is 1.7.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0ad431839783a9ac42a32d32b32c5a7d https://doi.org/10.1021/ac990422v Zobrazit plný text záznamu