Scanning Electron Microscopy of the Male Nematode Physaloptera (Tetradelphynema)
| Autor: | Randall E. McCoy, Ramesh K. Nayak, Bahja I. Behbehani |
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| Rok vydání: | 1977 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 35:674-675 |
| ISSN: | 2690-1315 0424-8201 |
| Popis: | Nematodes are elongate, unsegmented worms with an elastic cuticle made of protein. Few published references concerning scanning electron microscopy of nematode are available and there is paucity of information at the ultrastructural level on cuticle forming male reproductive structures. The species of the genus Physaloptera (tetradelphynema) commonly live as parasites in the adult form in the stomach of the desert rodent Gerbillus cheesmani. To date, the three-dimensional surface features of this spiruroid nematode have not been described. The main purpose of this investigation was to elucidate the surface features of the male nematode utilizing the current techniques of scanning electron microscopy.Adult worms were collected in the laboratory from the stomach of the gerbil, Gerbillus cheesmani. They were washed in PO4 buffer solution and subsequently fixed overnight in 2.5% glutaraldehyde in 0.1 M cacodylate buffer. Following fixation, the specimens were washed 3 times in buffer and sonically cleaned for 30 seconds to remove debris adhering on the body wall of the nematode. |
| Databáze: | OpenAIRE |
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