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This protocol explains how to use the GoTaq Dna polymerase kit from Promega to perform colony PCR. "GoTaq® DNA Polymerase contains GoTaq® DNA Polymerase, 5X Green GoTaq® Reaction Buffer and 5X Colorless GoTaq® Reaction Buffer. The enzyme is a Taq DNA polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The 5X Green GoTaq® Reaction Buffer contains two dyes (blue and yellow) that separate during electrophoresis to monitor migration progress. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR. Both buffers contain MgCl2 at a concentration of 7.5mM for a final concentration of 1.5mM in the 1X reaction." -Promega instructions |
