Detection of Benzodiazepines in Different Tissues, Including Bone, Using a Quantitative ELISA Assay
| Autor: | F. J. Melbye, Laura Y. Gorczynski |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Benzodiazepine
Pathology medicine.medical_specialty Chemistry medicine.drug_class Elisa assay Pharmacology Bone tissue Pathology and Forensic Medicine medicine.anatomical_structure Pharmacokinetics Genetics medicine Midazolam heterocyclic compounds Intramuscular injection Diazepam Clearance medicine.drug |
| Zdroj: | Journal of Forensic Sciences. 46:15069J |
| ISSN: | 0022-1198 |
| DOI: | 10.1520/jfs15069j |
| Popis: | Benzodiazepines were analyzed in different tissue samples, including hone, by ELISA. The sensitivity of detection for different benzodiazepines was consistent with the manufacturer's reports of the cross reactivities of the antibodies used, with the greatest sensitivity for midazolam and the least for diazepam; in addition the pharmacokinetics was consistent with the known duration of action of the different benzodiazepines, with midazolam cleared rapidly, and diazepam slowly. Following intramuscular injection of 300 microg of midazolam at 16 h intervals for ten days, the drug was detectable in bone tissue samples obtained from skeletonized remains buried in soil at room temperature for three weeks. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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