| Autor: |
Ji Yeun Kim, Bonhan Koo, So Yun Lim, Hye Hee Cha, Min Jae Kim, Yong Pil Chong, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim, Yong Shin, Sung-Han Kim |
| Rok vydání: |
2022 |
| DOI: |
10.21203/rs.3.rs-2039343/v1 |
| Popis: |
Background: Present molecular and microbiologic diagnostic tests have suboptimal sensitivity for diagnosing pauci-bacillary tuberculosis (TB). We recently developed a combined molecular and immunodiagnostic test for active TB involvingdetection of cell-free DNA (cfDNA) and of IFN-γ/TNF-α release from blood. We evaluated the diagnostic performance of this new test from patients with suspected TB. Methods: Adult patients suspected of active TB were prospectively enrolled from March 2019 to September 2021. CfDNA detection and the IFN-γ/TNF-αdual release fluorospot assay were performed using plasma and PBMC specimens. Final diagnosis of patients with suspected TB was provided by clinical category. Results: A total of 105 patients including 54 with active TB and 51 with not-active TB were finally analyzed. The sensitivity and specificity of cfDNA detection in plasma were 67% (95% CI, 53–79) and 80% (69–92), respectively, and those of the IFN-γ/TNF-αdual release fluorospot assay of PBMCs were 80% (67–89) and 90% (79–97), respectively. The sensitivity and specificity of the combined assays were 91% (80–97) and 77% (63–87). Conclusion: The combination of cfDNA detection and the IFN-γ/TNF-αdual release assay using blood specimens appears to be a useful adjunct test for the diagnosis of active TB. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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