Zinc deficiency in rat does not alter the major histone patterns in liver nuclei
| Autor: | C. Elizabeth Castro, Pei-Ting Cheng |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Nutrition and Dietetics
Euglena gracilis biology ved/biology Endocrinology Diabetes and Metabolism ved/biology.organism_classification_rank.species chemistry.chemical_element Zinc biology.organism_classification medicine.disease Euglena Chromatin Cell nucleus Endocrinology medicine.anatomical_structure Histone Biochemistry chemistry Hepatocyte medicine Zinc deficiency biology.protein |
| Zdroj: | Nutrition Research. 13:541-548 |
| ISSN: | 0271-5317 |
| DOI: | 10.1016/s0271-5317(05)80681-5 |
| Popis: | Zinc deficiency exerts many effects on DNA structure, metabolism and function. Others have observed that in the single-celled Euglena gracilis , zinc deficiency causes the distinctive appearance of a class of 3–5-kilodalton (kDa) polypeptides. The major histones, excepting H4, are released from chromatin of zinc deficient Euglena only when these 3–5-kDa polypeptides are removed. The purpose of this study was to determine whether zinc deficiency in rat is associated with a similar class of nuclear polypeptides and the accompanying increased affinity of the major histones to chromatin. Proteins were extracted from purified liver nuclei by two methods, 0.25 M hydrochloric acid (HCl) or 5% perchloric acid (PCA). There was no evidence of a class of 3–5-kDa polypeptides from nuclei of zinc deficient rats. There was also no qualitative change in the major histones. Although there were significant decreases in the amount of HCl and PCA-extracted proteins (P |
| Databáze: | OpenAIRE |
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