Simplified method of preparation of ovarian hormone, and properties of purified product

Autor:	C. N. Jordan, Edward A. Doisy, J. O. Ralls
Rok vydání:	1926
Předmět:	chemistry.chemical_compound Chromatography chemistry Cholesterol Bicarbonate Liquor folliculi Acetone lipids (amino acids peptides and proteins) Alcohol Petroleum ether Fraction (chemistry) General Biochemistry Genetics and Molecular Biology Saponification
Zdroj:	Experimental Biology and Medicine. 23:592-593
ISSN:	1535-3699 1535-3702
DOI:	10.3181/00379727-23-3071
Popis:	A detailed study of our former procedure for the preparation of the ovarian hormone from liquor folliculi has shown that several time-consuming steps may be omitted. Tests have indicated that the acetone precipitation of phospholipins may be dispensed with, since the bicarbonate of the liquor folliculi furnishes sufficient alkali to saponify the lipins during the concentration of the alcoholic extract.Another decided improvement has been effected by a study of the distribution ratios of the hormone and cholesterol between 70 per cent alcohol and petroleum ether. The cholesterol is twenty-nine times as soluble in petroleum ether as in 70 per cent alcohol. A few extractions of the alcoholic solution of the hormone with petroleum ether almost quantitatively remove the cholesterol but only a relatively insignificant fraction of the hormone.The procedure now advocated is designed to separate the hormone from the soaps and cholesterol. It is the following: The alcoholic extract of fresh liquor folliculi is dist...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0a7f5ff616f1a20e3072e6b80b04e72e https://doi.org/10.3181/00379727-23-3071 Zobrazit plný text záznamu