| Autor: |
Xueqing Wang, Quanlong Jiang, Hongdao Zhang, Zhidong He, Yuanyuan Song, Yifan Chen, Na Tang, Yifei Zhou, Yiping Li, Adam Antebi, Ligang Wu, Jing-Dong Han, Yidong Shen |
| Rok vydání: |
2023 |
| DOI: |
10.21203/rs.3.rs-2497512/v1 |
| Popis: |
Ageing in various tissues exhibits common and distinct features. It is critical to decipher the mechanisms underlying ageing in individual tissues. MicroRNAs (miRNAs) are essential regulators in ageing and are recently highlighted as a new class of intercellular signalling molecules. However, little is known about the tissue-specific transcriptomic changes of miRNAs during ageing. C. elegans is a well-established model organism in ageing research. In this study, we isolated five major somatic tissues in young and aged worms and profiled the age-dependent miRNAomic changes within these tissues. In addition to the diverse ageing-regulated miRNA expression across tissues, we discovered numerous miRNAs in the tissues without their transcription. We further profiled miRNAs in the extracellular vesicles (EVs) and found that worm miRNAs undergo inter-tissue trafficking via EVs in an age-dependent manner. Using these datasets, we uncovered the interaction between mir-1 from body wall muscle and DAF-16/FOXO in the intestine, suggesting mir-1 as a messenger in inter-tissue signalling. Taken together, our study systematically investigated miRNAs in the somatic tissues and EVs during ageing, providing a valuable resource to study tissue-autonomous and nonautonomous functions of miRNAs in ageing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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