A new lipase as a pharmaceutical target for battling infections caused byStaphylococcus aureus: Gene cloning and biochemical characterization
| Autor: | Ayhan Çelik, Aziz Tanriseven, Aişe Ünlü, I. Yavuz Sezen |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_treatment
Biomedical Engineering Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Affinity chromatography Drug Discovery medicine Lipase Escherichia coli chemistry.chemical_classification Protease biology Process Chemistry and Technology General Medicine Farnesol Enzyme Biochemistry chemistry Staphylococcus aureus biology.protein Molecular Medicine Coagulase Biotechnology |
| Zdroj: | Biotechnology and Applied Biochemistry. 62:642-651 |
| ISSN: | 0885-4513 |
| DOI: | 10.1002/bab.1316 |
| Popis: | Staphylococcus aureus lipases along with other cell-wall-associated proteins and enzymes (i.e., catalase, coagulase, protease, hyaluronidase, and β-lactamase) play important roles in the pathogenesis of S. aureus and are important subject of drug targeting. The appearance of antibiotic-resistant types of pathogenic S. aureus (e.g., methicillin-resistant S. aureus, MRSA) is a worldwide medical problem. In the present work, a novel lipase from a newly isolated MRSA strain from a cow with subclinical mastitis was cloned and biochemically characterized. The mature part of the lipase was expressed in Escherichia coli and purified by nickel affinity chromatography. It displays a high lipase activity at pH 8.0 and 25 °C using p-nitrophenyl palmitate and has a preference for medium-long-chain substrates of p-nitrophenyl esters (pNPC10-C16). Furthermore, in search of inhibitors, the effect of farnesol on the growth of S. aureus and the lipase activity was also studied. Farnesol inhibits the growth of S. aureus and is a mixed-type inhibitor with Ki and Ki (') values of 0.2 and 1.2 mmol L(-1), respectively. A lipase with known properties could not only serve as a template for developing inhibitors for S. aureus but also a valuable addition to enzyme toolbox of biocatalysis. The discovery of this lipase can be potentially important and could provide a new target for pharmaceutical intervention against S. aureus infection. |
| Databáze: | OpenAIRE |
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