Regeneration of Plants from Protoplasts ofArabidopsis thalianaL. cv. Columbia (C24), Via Direct Embryogenesis
| Autor: | C. M. O'neill, R. J. Mathias |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
biology Cell division Somatic embryogenesis Physiology Regeneration (biology) fungi food and beverages Plant Science biochemical phenomena metabolism and nutrition Protoplast biology.organism_classification Micropropagation chemistry Auxin Arabidopsis Botany bacteria Arabidopsis thaliana |
| Zdroj: | Journal of Experimental Botany. 44:1579-1585 |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/44.10.1579 |
| Popis: | This report describes a protocol for the regeneration of fertile plants from mesophyll protoplasts of Arabidopsis Arabidopsis thaliana race Columbia (C24). Regeneration was rapid and reproducible. The protocol is especially novel in that a large proportion of regenerating protoplasts regenerated via direct somatic embryogenesis. Protoplasts isolated from in vitro-grown plants entered sustained division after 3-5 d in culture medium and over a period of several days 6-22% of protoplasts underwent at least one cell division. Approximately 2-16% of these protoplasts continued to divide and after 3 weeks in culture had formed macroscopic colonies, of which 70-80% were regular embryo-like structures |
| Databáze: | OpenAIRE |
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