| Popis: |
This study is aimed to search the possibility of developmental toxicity test using the zebrafish fromthe pesticide. We selected herbicides alachlor and butachlor, reported for fish toxicity, and insecticide fipronilreported for the high fish toxicity and the honey bee risk among the pesticides with high usability for theexamples of the pesticides in this experiment. In this study, we showed those effects on the zebrafish embryodevelopment by exposing different kinds of pesticide with different concentration and exposed time periods. As aresult, the rates of hatching and abnormality of the zebrafish embryo after treatments of alachlor were increased in24-48 hpf group, and the juvenile fishes in every group exposed to 40 µM or more of alachlor displayed severmorphological changes such as bent tails, edema and activity failures. In case of the butachlor, the rates ofhatching and the abnormality in 24-48 hpf group were higher than the other groups exposed in different timeperiods. The fatality before hatching was high in 40 µM or more of butachlor treatment, and entire zebrafishembryos in 48 hpf group died before hatching. All the living juvenile fishes showed morphological changes aslike as the treatment of alachlor. The rate of hatching and the survival of the zebrafish embryo by the fipronilwere higher than other pesticides. However, morphological changes such as bent tails were observed from themost of living juvenile fishes. Therefore, the effects of three different pesticides with different concentrations andexposing time periods on the development of zebrafish embryos showed that all the pesticides effects wereproportional to the concentration, and exposing time periods may cause the morphological abnormality.Key words alachlor, butachlor, developmental toxicity test, fipronil, Zebrafish embryo |
